Table 6.
Cost Comparison between MALDI-TOF Genotyping and Conventional Methods Used for HBB Genotyping in this Study
| MALDI-TOF genotyping |
Conventional method |
|||||
|---|---|---|---|---|---|---|
| 1 Panel | 2 Panels | 3 Panels | DGGE | RFLP | DNA Sequencing | |
| Number of detectable mutations | 21 | 30 | 30* | 18 | 1 (Hb E) | 11† |
| Percent total HBB alleles in Thai population | 95% | 99.5% | 99.5* % | 65% | 30% | 4.5% |
| Analysis time (h) | 22 | 22 | 22 | 17 | 22 | 14 |
| Reagent cost ($) | ||||||
| DNA preparation | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| PCR | 1.25 | 1.25 | 1.25 | 7.50 (1.25 × 6) | 1.25 | 7.50 (1.25 × 6) |
| Post-PCR | 3.75 | 7.50 | 11.25 | − | 0.75 | 52.50 |
| Analysis step | 2.00 | 4.00 | 6.00 | 2.50 | − | 7.50 |
| Total cost | 12.00 | 17.80 | 23.50 | 15.00 | 7.00 | 72.50 |
| Cost ($) per mutation | 0.57 | 0.59 | 0.78 | 0.83 | 7.00 | 6.60 |
| Average for 30 mutations = 0.69 | Average for 30 mutations = 3.15 | |||||
*
Optional for specific confirmation of c.20A>T (Hb S), c.52A>T, and c.441_442insAC.
†
HBB mutations requiring DNA sequencing analysis are c.−137C>A, c.2T>G, c.5T>C (Hb Raleigh), c.19G>A (Hb C), c.20A>T (Hb S), c.20A>C (Hb G Makassar), c.46delT, C.251G>A (Hb Pyrgos), c.364G>C (Hb D Punjab), c.380T>G (Hb Dhonburi), and c.410G>A (Hb Hope).