Figure 1.

RealTime linearity and reproducibility. Serial dilutions of four high-titer clinical samples (IH03, IH06, IH11, and IH12) were prepared, five replicates each, and tested in the 1 ml RealTime assay. Three replicates were tested on a single run to evaluate intra-assay reproducibility, and the remaining two replicates were tested on a second and third run (and compared with the mean of the intra-assay data) to evaluate interassay reproducibility. The expected values were calculated by setting the concentration with the lowest coefficient of variation at identity, and using the dilution factor to calculate the remaining expected concentrations. Replicates that were below the assay's quantification limit are not shown.