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Comparison of UL97 mutation analysis by LightCycler PCR (A-C) and RFLP assay (D-F) of sequential DNA-specimens from blood of a pediatric HSCT recipient with GCV-resistance based on UL97 mutation C603W. Specific melting point analysis and restriction patterns of the investigated patient DNA in comparison with the laboratory wild-type strain AD 169 and a clinical reference strain (5966) or plasmid (pC603W) containing the mutation C603W are shown.
