Table 1.

Study design (n = 14)

Day	Group 1	Group 2	Group 3
0	Bilateral oophorectomy	Bilateral oophorectomy	Sham operation
15, 16, and 22	5 mg/0.1 mL E2 in sesame oil, SC	0.1 mL sesame oil, SC	0.1 mL sesame oil, SC
23	Bile duct ligation	Bile duct ligation	Bile duct ligation
37	Killed, blood and tissue samples obtained	Killed, blood and tissue samples obtained	Killed, blood and tissue samples obtained

SC: Subcutane.