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. 2008 Nov;28(10):1446–1457. doi: 10.1111/j.1478-3231.2008.01759.x

Fig. 2.

Fig. 2

Effect of platelet-derived growth factor β subunit (PDGFR-β) short hairpin RNA (shRNA) on the proliferation of hepatic stellate cells (HSCs). (A) showed statistical results of bromodeoxyuridine incorporation detected by immunofluorescence (IF) and flow cytometry (FACs) in HSCs without treatment (HSC), HSCs with platelet-derived growth factor (PDGF)-BB (HSC+PDGF), HSCs with pCMV–NC–LacZ and PDGF-BB (HSC+PDGF+RNAcon) and HSCs with pCMV–shRNA–LacZ and PDGF-BB (HSC+PDGF+RNA interference). The results revealed that PDGF-BB significantly promoted the proliferation of HSCs (P=0.008 with flow cytometry analysis and P=0.012 with immunofluorescence), which was inhibited by pCMV–shRNA–LacZ (P=0.003 with flow cytometry analysis and P=0.006 with immunofluorescence). *P<0.05, **P<0.01. (B) shows the representative graph of extracellular-signal-related kinase (ERK) phosphorylation in HSCs without treatment, HSCs with PDGF-BB, HSCs with pCMV–NC–LacZ and PDGF-BB, HSCs with pCMV–shRNA–LacZ and PDGF-BB and HSCs with PD98059 and PDGF-BB by Western blot analysis. PD98059 is a specific inhibitor of ERK phosphorylation.