Table 1.
The IS‐ASC response in the spleen and bone marrow after influenza H7N1 vaccination of BALB/c mice
| Organ | Antibody class/IgG subclass | Days post first vaccination | Days post second vaccination | ||||
|---|---|---|---|---|---|---|---|
| 0 | 5 | 7 | 3 | 5 | 21 | ||
| Spleen | IgM | 4·4 ± 0·6 | 28·9 ± 10·8 | 9·1 ± 6·5 | 14·1 ± 6·0 | 9·1 ± 2·4 | 8·2 ± 2·5 |
| IgA | < | < | < | 9·2 ± 3·6 | 15·7 ± 4·2 | 0·3 ± 0·3 | |
| IgG | 0·6 ± 0·6 | 0·2 ± 0·2 | < | 33·5 ± 10·9 | 32·4 ± 6·2 | 3·0 ± 1·4 | |
| IgG1 | < | < | < | 5·7 ± 3·2 | 3·8 ± 2·0 | 1·0 ± 0·6 | |
| IgG2a | < | 0·2 ± 0·2 | < | 30·9 ± 8·1 | 19·7 ± 3·7 | 0·4 ± 0·2 | |
| IgG2b | < | 1·1 ± 0·5 | < | 8·3 ± 3·2 | 7·0 ± 1·2 | 0·5 ± 0·5 | |
| IgG3 | < | < | < | 5·0 ± 3·8 | 0·5 ± 0·5 | 0·5 ± 0·3 | |
| Bone marrow | IgM | 0·8 ± 0·6 | 4·4 ± 1·4 | 3·9 ± 1·4 | 3·9 ± 1·4 | 7·4 ± 5·7 | 1·9 ± 0·8 |
| IgA | < | < | < | 0·6 ± 0 | 3·8 ± 1·4 | < | |
| IgG | < | < | < | 1·4 ± 0·4 | 7·8 ± 2·5 | 1·9 ± 0·6 | |
| IgG1 | < | < | < | 0·3 ± 0·3 | 0·3 ± 0·2 | 1·1 ± 0·5 | |
| IgG2a | < | < | < | 3·1 ± 1·2 | 1·6 ± 0·4 | 0·2 ± 0·2 | |
| IgG2b | < | < | < | 1·0 ± 0·4 | 0·8 ± 0·4 | 0·2 ± 0·2 | |
| IgG3 | < | < | < | < | 0·2 ± 0·2 | 0·5 ± 0·5 | |
<no specific IS‐ASC detected per 500 000 lymphocytes.
BALB/c mice were immunised with one or two doses of whole virus vaccine (15 μg total protein). Groups of four mice were sacrificed after vaccination or control unimmunised mice (0) and the antibody secreting cell response measured by ELISPOT assay. The mean number of influenza H7N1 specific antibody secreting cells per 500 000 lymphocytes ± standard error of the mean (SEM).