Fig. 4.

Measurement of protein tyrosine phosphorylation in Gads-deficient platelets. Washed mouse platelets (5 × 10⁸ mL⁻¹) were stimulated with CRP for 60 s (A) or rhodocytin for 120 s (B) and subsequently lysed with NP-40 detergent. PLCγ2, SLP-76, and Syk were immunoprecipitated using pAbs and proteins were separated by reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (10%) and transferred to polyvinylidene fluoride membrane. The membrane was probed with an anti-pTyr mAb, and reprobed with PLCγ2, SLP-76 or Syk pAbs. Results are representative of between four and six experiments.