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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: J Neurochem. 2009 Mar 20;109(5):1300–1310. doi: 10.1111/j.1471-4159.2009.06048.x

Figure 8. UTP-stimulated sAPPα release in IL-1β-treated rPCNs is partially dependent on ERK1/2 activation.

Figure 8

Figure 8

rPCNs were pretreated with 25 ng/ml IL-1β in serum-free DMEM for 24 h at 37 °C. The medium was replaced with fresh serum-free DMEM and the cells were incubated at 37 °C for 30 min with or without U0126 (1 µM) followed by a 2 h incubation with or without UTP (100 µM). (A) The release of sAPPα into the medium was analyzed by Western blot analysis. (B) sAPPα release is expressed relative to the actin level in cell lysate. Data represent the means ± S.E.M. of results from three experiments, where * represents significant differences (p < 0.05) between the indicated sample groups analyzed by 2-way ANOVA followed by Bonferroni post-tests.