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The chc1-box allele, but not chc1-TD-ts alleles, rescues the endocytic phenotype of chc1Δ. MATa chc1Δ bar1-1 (SL3593) was transformed with pUN30 (CEN, empty), pAP4 (CEN, CHC1), pJRC2 (CEN, chc1-1), pJRC3 (CEN, chc1-2), pJRC4 (CEN, chc1-3), pJRC5 (CEN, chc1-4), or pJRC19 (CEN, chc1-box). α-factor internalization was analyzed at 37°C as described in Materials and Methods.
