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. 2009 Jul 15;20(14):3401–3413. doi: 10.1091/mbc.E08-10-1082

Figure 5.

Figure 5.

GFP-Clc1p accumulation at the cell cortex in chc1-TD alleles in the presence of LAT-A. GFP-CLC1 ABP1-mRFP chc1Δ (SL5538) was transformed with pAP4 (CEN, CHC1), pJRC2 (CEN, chc1-1), pJRC3 (CEN, chc1-2), pJRC4 (CEN, chc1-3), pJRC5 (CEN, chc1-4), or pJRC19 (CEN, chc1-box). Cells grown to log phase at 25°C were treated with LAT-A (250 μM) for 20 min at 25°C before imaging. Abp1-mRFP was completely cytosolic after 20 min in LAT-A, indicating the disassembly of actin (data not shown). Arrows indicate the accumulation of clathrin at the cell cortex. Cells that showed strong cortical puncta or rim GFP staining were considered to have accumulated clathrin. The percentage of cells for each condition that contained cortical GFP-Clc1 accumulation is shown in the graph (n = 100–150).