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. 2009 Jul 15;20(14):3436–3450. doi: 10.1091/mbc.E08-03-0325

Figure 3.

Figure 3.

Smurf1 regulates endogenous TRAF4. (A) Western blot of Smurf1 and TRAF4 proteins from HeLa cells transfected with control or Smurf1-specific siRNA. Partial reduction of Smurf1 protein resulted in elevated TRAF4. (B) Quantitation of Smurf1 and TRAF4 proteins detected in the Western blot. Smurf1-specific siRNA reduced Smurf1 proteins levels by as much as 55% compared with control siRNA, resulting in a corresponding 55–75% increase in TRAF4. Expression levels are normalized to the average of control triplicates.