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. 2009 Jul 15;20(14):3261–3272. doi: 10.1091/mbc.E09-01-0071

Figure 3.

Figure 3.

PM area increase is due to the balance between exocytosis and endocytosis. (A) Schematic representation of the FM1-43 during spreading protocol (see Materials and Methods). (B) RPTPα cells using the FM1-43 during spreading protocol. Arrow, perinuclear region brightening showing endocytosis. Bar, 10 μm. (C). FM1-43 fluorescence intensity (-fold increases, compared with the initial FM1-43 fluorescence intensity at t = 0″) of each cells plotted as a function of time. (D) Same as B, but without wash and for cells presenting a significant difference in size. (E) FM1-43 fluorescence intensity and fluorescence intensity -fold increases plotted as a function of time for each cell presented in D. (F) Substrate contact area plotted in function of the initial PM area (represented by the FM1-43 fluorescence intensity at t = 0″) after 30 min of spreading for RPTPα cells (red dots and red fitting line; n = 10) and 60 min of spreading for NIH-3T3 cells (blue dots and blue fitting line; n = 10).