Figure 2.

Synthetic dosage phenotype caused by overexpression of BNI4 in the absence of G1 Cdks. (A) Cultures of WT cells carrying pGAL-GST-BNI4, cln1Δcln2Δ carrying vector (pEGH), cln1Δcln2Δ carrying pGAL-GST-BNI4, pcl1Δpcl2Δ carrying vector (pEGH), and pcl1Δpcl2Δ carrying pGAL-GST-BNI4 were serially diluted and spotted on both SD-ura and SG-ura plates. Plates were incubated 2 d for both SD-ura and SGal-ura plates at 30°C before being photographed. (B) Cultures were grown to log phase in SD-ura, and then transferred to SR-ura and incubated for another 5 h. Galactose was then added to 2%, and cultures were incubated at 30°C for an additional 4.5 h and examined by microscope. Bar, 10 μm.