Figure 5.

Schematic of Myo4p–She2p chimeras and Myo4p dimers. (A) The Myo4p head is directly joined to the mRNA binding protein She2p, thus bypassing the requirement for the rod and She3p. (B) The Myo4p head, or full-length Myo4p, is joined to two copies of She2p. The tandem copies of She2p are separated by a poly-Gly₁₀ linker to allow intramolecular dimerization. These constructs ensure one motor head per She2p dimer. (C) Constitutive dimers were engineered by adding a leucine zipper immediately after the predicted α-helical coiled coil region in either a construct lacking the GT, or full-length Myo4p.