Temperature-dependence of infrared changes in a hydrated phosphatidylcholine film at its main phase transition. A: symmetric methylene stretch frequency; B: antisymmetric methyl stretch frequency; C: antisymmetric methylene stretch frequency, respectively. D shows line-width change for the antisymmetric methylene stretch. Solid lines are the fits (r2≥ 0.994) of Eq. 1 to the data points (open circles), which yielded the following parameters: for symmetric methylene stretch (A), νRT = 2853.1 cm−1, Δν=3.0 cm−1; for antisymmetric methyl stretch (B), νRT = 2923.1 cm−1, Δν=4.5 cm−1; for antisymmetric methylene stretch (C), νRT = 2959.3 cm−1, Δν=2.7 cm−1; and for narrowing of the antisymmetric methylene stretch band characterized by its full width at half maximum, FWHM, (D), FWHMRT = 25.3 cm−1, ΔFWHMRT = 5.6 cm−1.