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. 2000 Sep 19;97(20):10832–10837. doi: 10.1073/pnas.170276797

Figure 1.

Figure 1

Binding of Akt to Hsp90 in vivo. (A) BALB/3T3 and 293T cell lysates were incubated with protein G agarose conjugated with control sheep IgG or sheep anti-Akt pAb. (B) 293T cells were transfected with mock or the FLAG-tagged WT-akt. The cell lysates were incubated with an anti–FLAG M2 agarose. (C) 293T cells were cotransfected with the V5-tagged WT-hsp90β and the indicated FLAG-tagged plasmids. The cell lysates were incubated with an anti–FLAG M2 agarose. The immunoprecipitated proteins were immunoblotted with the indicated antibodies. Molecular size markers are indicated (in kDa).