Fig. 6.

Effect of citric acid desorption on haematoxylin staining (A) and JIM7-recognized HG epitope immunofluorescence labelling (B). Three-day-old seedlings (‘Nongda3138’) were subjected to 0·5 mm CaCl₂ solution (pH 4·5) either with 0 (–Al) or 100 µm Al for 9 h. After treatment, roots were washed with 0·5 mm CaCl₂ solution at pH 4·5 (+Al + Ca) or with 1 mm citric acid in 0·5 mm CaCl₂ solution at pH 4·5 (+Al + Cit) for 30 min. After desorption, roots were used for haematoxylin staining and immunolabelling. The images are representative of at least ten replicate roots for each treatment. Scale bars = 50 µm.