Table 2.

mRNA fold increase above levels expressed by undifferentiated CD34⁺ cells

	Day 7	Day 14
PAFR Tr1	1.6	1.8
PAFR Tr2	ND	ND

qRT-PCR of PAF receptor isoforms during megakaryopoiesis. mRNA for PAFR-1 (PAFR Tr1) and PAFR-2 (PAFR Tr2) was quantified by qRT-PCR and relative values of megakaryocyte precursors and differentiated megakaryocytes were compared with culture day 0 (CD34⁺ cells) by the 2^−ΔΔCt method.³⁴ mRNA for PAFR-1 was normalized to β-actin transcript levels. PAFR-2 was not detected (ND) in CD34⁺ cells, megakaryocyte precursors, or megakaryocytes (also see Figure S4).