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. 2009 Apr 17;113(25):6392–6402. doi: 10.1182/blood-2009-03-209650

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© 2009 by The American Society of Hematology

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Expression and improved migration of CCR4⁺ T cells established from persons affected by HL. (A) The expression of CCR4 by CTR () and transduced (■) T cells established from 4 persons with HL. The data are mean ± SD. (B) A representative phenotypic analysis. (C) The migration of CTR () and CCR4⁺ (■) T cells toward TARC gradients, using a transwell migration assay. T-cell migration was evaluated using culture supernatants collected from HDLM-2 and L428, which physiologically produce high amounts of TARC, and against Karpas genetically modified to produce TARC (K/TARC). K/wt represents migration toward Karpas wild-type, which produces a negligible amount of TARC, as a negative control. Migration is significantly increased for CCR4⁺ T cells compared with CTR T cells. In addition, the figure shows that the improved migration of CCR4⁺ T cells (■) is TARC mediated as it is inhibited by addition of anti-TARC antibodies but not by addition of an isotype control.

Inline graphic — Expression and improved migration of CCR4⁺ T cells established from persons affected by HL. (A) The expression of CCR4 by CTR () and transduced (■) T cells established from 4 persons with HL. The data are mean ± SD. (B) A representative phenotypic analysis. (C) The migration of CTR () and CCR4⁺ (■) T cells toward TARC gradients, using a transwell migration assay. T-cell migration was evaluated using culture supernatants collected from HDLM-2 and L428, which physiologically produce high amounts of TARC, and against Karpas genetically modified to produce TARC (K/TARC). K/wt represents migration toward Karpas wild-type, which produces a negligible amount of TARC, as a negative control. Migration is significantly increased for CCR4⁺ T cells compared with CTR T cells. In addition, the figure shows that the improved migration of CCR4⁺ T cells (■) is TARC mediated as it is inhibited by addition of anti-TARC antibodies but not by addition of an isotype control.