Figure 4.

Atg7-independent mitochondrial clearance is mediated by degradative vacuoles. (Top) Ultrastructure of reticulocytes from Atg7^+/+ and Atg7^−/− transplant recipients cultured for 1 day. The scale is indicated at the bottom. (Bottom) Quantification of mitochondria and degradative vacuoles in cultured Atg7^+/+, Atg7^−/−, and Nix^−/− reticulocytes expressed as a ratio. Fifty digital images containing 100 to 200 mitochondria and vacuoles per sample were quantified. Genotypes and days in culture are indicated at the bottom.