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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: J Allergy Clin Immunol. 2008 Oct 11;122(6):1169–1177.e16. doi: 10.1016/j.jaci.2008.08.018

Figure 4. Expression levels of reconstituted NEMO are equivalent by anti-NEMO Western blot, intracellular FACS, and GFP FACS.

Figure 4

A, Cells from reconstituted lines were lysed and probed with anti-NEMO monoclonal antibody specific for the C-terminus. Actin blotting demonstrates equal loading. B, FACS to determine GFP expression was performed on NEMO reconstituted cells lines, C, which was evaluated by intracellular staining (n=2).