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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Neurochem. 2009 Feb;108(4):1019–1031. doi: 10.1111/j.1471-4159.2008.05851.x

Figure 3. Deletions in the globular domain of CyPrP eliminate PrP’s anti-Bax function.

Figure 3

A & B. Schematic diagram of the sequential C-terminal CyPrP deletion mutants showing the main domains and structural elements. Right panel: Percentage (mean and SEM) of condensed chromatin positive cells in transfected MCF-7 cells. Data represents results from 4 to 5 independent experiments. At least 100 cells were counted for each experiment. Asterisk indicates a statistically significant difference from pBud-EGFP or pBud-EGFP-Bax (p≤0.05). C. Western blot analyses with 3F4 and β-actin antibodies of proteins extracted from cells transfected with CyPrP or CyPrP C-terminal globular domain deletion mutants. D. Western blot of total protein extracts from CyPrP23-231- or CyPrP23-227-transfected cells with 3F4, anti-GFP and anti-β-actin antibodies.