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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Neurochem. 2009 Feb;108(4):1019–1031. doi: 10.1111/j.1471-4159.2008.05851.x

Figure 5. Effect of PrP helix 3 on the anti-Bax function of PrP in MCF-7 cells.

Figure 5

A. Schematic diagram of the helix 3 construct relative to the full length CyPrP. B. Western blot with anti-His Tag and anti-β-actin antibodies. Lower panel shows Coomassie blue stain of the area depicted in the western blots. C. Percentage cell death assessed by condensed chromatin in MCF-7 cells transfected with pBud-EGFP or pBud-EGFP/CyPrP, or /PrP helix 3 tag, and pBud-EGFP-Bax, pBud-EGFP-Bax/CyPrP or /PrP helix 3 tag. Data represents the mean ± SEM of 7 independent experiments. At least 100 cells were counted in each experiment. Asterisk indicates a statistically significant difference from pBud-EGFP or pBud-EGFP-Bax-transfected cells (p≤0.05). D. Percentage of cells with caspase activity assessed by FLICA in MCF-7 cells transfected with pBud-EGFP or pBudEGFP/CyPrP or /PrP helix 3 tag and pBud-EGFP-Bax or pBud-EGFP-Bax/CyPrP or /PrP helix 3 tag. Data represents the mean ± SEM of 3 independent experiments. At least 100 cells were counted in each experiment. Asterisk indicates a statistically significant difference from pBud-EGFP or pBud-EGFP-Bax (p≤0.05).