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. 2000 Sep 26;97(20):10838–10843. doi: 10.1073/pnas.97.20.10838

Figure 1.

Figure 1

sqv-3+ restores FGF-2 binding in mutant pgsB-618 cells. A stable cell line was derived from Gal transferase I-deficient mutant (pgsB-618) by stable transfection with sqv-3+ cDNA. Wild-type, mutant, and transfected cells were incubated at 37°C and treated with biotinylated FGF-2 and FITC-labeled streptavidin before cell sorting (29). (Top) Wild-type CHO cells. (Middle) Mutant pgsB-618 cells. (Bottom) Mutant pgsB-618 transfected with sqv-3+. The shaded curve represents sorts done on cells without added biotin-FGF.