Figure 3.

Western blot analysis of chondroitin-modified proteoglycans in wild-type (N2), sqv-3, sqv-7, and sqv-8 homozygous L4 animals, detected with anti-ΔDi-0S antibody. (A) Analysis of wild-type proteoglycans, digestion with chondroitinase ABC after (lane 1) or before (lane 2) electrophoresis and blotting. Proteoglycans in lane 3 were not treated with chondroitinase, showing that enzymatic digestion is required for antibody detection of these molecules. (B) Comparison of chondroitin-modified proteoglycans from wild-type (lane 1), sqv-7 (lane 2), sqv-3 (lane 3), sqv-8 (lane 4) mutants, where chondroitinase digestion was performed after electrophoresis and blotting.