Table I.
Acceptor | GalT activity, pmol/h/assay* | GlcAT-I activity, pmol/h/assay* |
---|---|---|
Xylose-β-nitrophenyl | 82 ± 12 | |
GlcNAc-β-nitrophenyl | 57 ± 9 | |
GlcNAc-α-nitrophenyl | 37 ± 10 | |
Galβ1,3-Galβ-O-NM | 137.4 ± 19.8 | |
Galβ-O-2-naphthol | 8.6 ± 0.5 | |
Gal1,3β-GalNAc-α-1-O-benzyl | 11.8 ± 1 | |
Galβ1,3-GlcNAcβ-O-NM | 12.1 ± 0.8 | |
Galβ1,4-GlcNAcβ-O-NM | 11 ± 1.2 |
Values are means of two independent experiments run in duplicate. NM, naphthalenemethanol; assays were run for 1–3 h at the optimum temperature (17°C).