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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Methods Mol Biol. 2008;457:305–317. doi: 10.1007/978-1-59745-261-8_23

Fig. 2.

Fig. 2

Alexa488-labeled InlB internalization is blocked by hypertonic shock. T47D/cMet were cells preincubated in DMEM (− hypertonic) or containing 0.45 M sucrose (+ hypertonic) for 15 min at 37°C. The cells were then incubated in the corresponding media supplemented with 100 ng/mL of Alexa488-labeled InlB and 5 μg/mL Alexa 594-labeled Transferrin (Tfn) for 15 min at 37°C and then processed for confocal microscopy. Representative examples are shown (scale, 10 μm). Hypertonic treatment results in the colocalization of InlB and Tfn on the cell surface (closed arrows) consistent with the inhibition of cMet internalization. Conversely, internalized InlB colocalizes with internalized Tfn (open arrows) in non hypertonic cells and appear as punctate spots reminiscent of early endosomes.