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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: J Immunol. 2008 Jan 1;180(1):238–248. doi: 10.4049/jimmunol.180.1.238

FIGURE 1.

FIGURE 1

LTαβ-LTβR interaction mediates homeostasis of CD8− DC subsets. A, CD11chigh cells in the spleen were analyzed for CD4 and CD8α expression in wt B6, LTβR-, LTβ-, LTα-, LIGHT- and LTβ/LIGHT-deficient mice by flow cytometry as described in Materials and Methods. A representative histogram is shown for each mouse strain. The ratio of CD8α to CD4 DC subsets was calculated from values in the upper left and lower right quadrants. B, The percentage of DC are presented as a fraction of total nucleated splenocytes (top panel) and the total number of DC (bottom panel) in the spleen from the indicated gene deficient mice. Each data point represents an individual animal and the data are pooled from two analyses. C. The percentage (top panel) and total number (bottom panel) of individual CD4+, CD8α, and CD8α−/CD4− DC subsets within the gated CD11chigh DC. The bars are the mean ± SD from at least three mice per group and the data are representative of three independent experiments. In all panels, Student t test evaluation of significance where *, **, and *** denote p < 0.05, p < 0.01, and p < 0.001, respectively, between the indicated groups.