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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: J Immunol. 2008 Jan 1;180(1):238–248. doi: 10.4049/jimmunol.180.1.238

FIGURE 5.

FIGURE 5

LIGHT/LTβ/HVEM mice reveal an LT-independent DC subset with normal CD8α/CD4 subset ratio. A. CD11chigh cells gated as indicated were analyzed for CD4 and CD8α expression in WT B6, LTβ/LIGHT, and LTβ/LIGHT/HVEM-deficient mice as described in the Materials and Methods. A representative histogram is shown for each mouse strain. The ratio of CD8α to CD4 DC subsets was calculated from values in the upper left and lower right quadrants. B, The percentage of DC as a fraction of total nucleated splenocytes (top panel), the percentage of individual DC subsets (middle panel) and the total number cells in each DC subset (bottom panel) in the spleen from the indicated gene deficient mice were calculated from flow data. Each data point represents the value obtained from an individual animal and the data are pooled from two analyses. Bars show the mean ± SD from at least three mice per group, and the data are representative of three independent experiments. Student’s t test was performed where *, **, and *** denote significance of p < 0.05, p < 0.01, and p < 0.001, respectively, between the indicated groups.