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RT-PCR analysis of wild type (WT), Pro35S:ETR1-1 (R8, R14, R15, R25 and R31) and Pro35S:LeEIL1 (e1, e13, e17 and e 24) Nicotiana sylvestris lines. RNA was extracted from mature leaves and used to generate cDNA. Amplification was achieved using ETR1-1- (R8–WT) or LeEIL1- (e1–WT) specific primers.
