Figure 2. Apoptosis is normal in HtrA2 mutants.

(a-d) Confocal micrographs of the pouch region of wing imaginal discs stained with anti-cleaved caspase 3 (red) and phalloidin (green) after mock treatment or exposure to 4Gy γ-rays. No difference was detected between precise excision controls (a, b) and HtrA2^Δ1 mutants (c, d). Posterior is to the right. Scale bar = 50μm. (e) Quantification of the apoptotic index from wing discs after treatment with γ-rays (see materials and methods). n=10 for each genotype. (f) Quantification of the apoptotic index from wing discs after treatment with Staurosporine or UV light (see materials and methods). n=8 for each genotype. Error bars represent standard deviations.