Figure 5.

The anti-MEL-46 antibody is specific and the truncated protein is produced and stable in mel-46(yt5) mutants. Western blot analysis of protein extracts from wild type, mel-46(yt5), mel-46(yt5) rescued with a wild type copy expressed from a transgene and mel-46(tm1739) rescued with a wild type copy expressed from a transgene. All extracts are from worms that were raised at 25°C. Asterisks designate a band that is specific for worms that contain a wild type copy of mel-46, filled circles point to a band that is specific for worms with a mel-46(yt5) mutant copy. Both bands appear somewhat larger than predicted based on the protein sequence (110.4 kD and 103.1 kD respectively). The band around 110 kDa that is present in all lanes (arrow) is also detected by the pre-immune serum (data not shown) and serves here as an internal loading control.