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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2009 Jun 30;8(7):2015–2026. doi: 10.1158/1535-7163.MCT-08-0929

Figure 1.

Figure 1

Expression, subcellular localization and activity of Fpg-FLAG-NLS and α-OGG1-FLAG in stable HEK 293 clones. (a) detection of FLAG-tagged ectopic protein expression by immunocytochemistry (transient transfections). (b, c) stable expression of Fpg-FLAG-NLS (b) and α-OGG1-FLAG (c) in both nuclear (N) and mitochondrial (Mt) compartments, as observed with detection of ectopic proteins and FLAG tag by Western blotting. Membranes were re-probed for Proliferating Cell Nuclear Antigen (PCNA) to confirm purity of subcellular fractions. (d, e) in vitro 8-oxodG incision assay demonstrates increased 8-oxodG repair activity in nuclear and mitochondrial lysates from fpg (d) and α-OGG1 (e) clones. Values beneath sample lanes represent the ratio of cut (21mer) to uncut (49mer) substrate as determined by densitometry.