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. 2009 Apr 8;96(7):2799–2807. doi: 10.1016/j.bpj.2008.11.069

Figure 4.

Figure 4

The tail only weakly inhibits release of Mg2+ from the nucleotide pocket. Removal of Mg2+ from the nucleotide pocket is followed sequentially by rapid release of bound nucleotide. Normalized time-dependent traces of Mant-ADP release from K420 occurring after addition of EDTA + ATP are shown. Smooth lines are weighted first-order fits to the normalized average traces. Rates and standard deviations are shown below the curves (n = 5 for both samples). The rate of Mant-ADP release from EDTA-treated K420 was only marginally different in the absence and presence of Tail944.