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Illustration of the implementation of Lucky imaging for single molecule fluorescence. Δx, the precision in the 2D Gaussian fitting of the PSF for a Cy3B fluorophore, is plotted as a function of time where each of the time points is an image frame from a TIRF image stack recorded with an exposure time of 0.08 s per frame. The bold line shows the Lucky threshold applied to the data at 4.80 nm. The square data points show the retained data points.
