Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Data from a TIRF experiment of a single Cy3B molecule imaged for 1000 frames with an exposure time of 0.08 s per frame. The sigmoidal relationship between Δxrms and the applied Lucky threshold is shown. (B) Δxrms data for one fluorescent spot imaged for 300 frames at a variety of exposure times to assess the optimum exposure time for our apparatus. All the data are from the same Cy3B molecule. Δxrms decays exponentially (τ = 0.011) with exposure time.
