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. 2009 Apr 8;96(7):2832–2848. doi: 10.1016/j.bpj.2008.12.3941

Figure 8.

Figure 8

(A) ITC microcalorimetric traces and the derived isotherms of 25 μM CaM titrated with ∼500 μM Cx44129–150 in 20 mM PIPES, 100 mM KCl, 2 mM CaCl2, pH 6.8 at 25°C. (B) Comparison of the enthalpic (ΔH) and entropic (−TΔS) contributions to the Gibbs free energy (ΔG) on the formation of the holo-CaM-peptide complexes. Data from Brokx et al. (36).