Table 2.
Distances between spin labels labeled at cysteine residues, with the V49A mutation as background
| Doubly labeledmutants | Distances at nonilluminated state (Å) | Distances at illuminated state (Å) | |||
|---|---|---|---|---|---|
| V101R1/A168R1 | pH 6 | 8.6 ± 1.9 | 17.8 ± 1.2 | 8.6 ± 1.9 | 17.8 ± 1.2 |
| pH 9 | 8.4 ± 1.5 | 17.3 ± 1.5 | 8.0 ± 1.0 | 17.1 ± 1.8 | |
| L100R1/T170R1 | pH 6 | 8.2 ± 0.9 | 15.8 ± 2.6 | 8.0 ± 0.8 | 15.6 ± 2.9 |
| pH 9 | 8.1 ± 0.8 | 15.7 ± 2.5 | 8.5 ± 0.9 | 15.8 ± 2.6 | |
| S169R1/S226R1 | pH 6 | 9.0 ± 3.1 | — | 9.2 ± 3.0 | — |
| pH 9 | 9.5 ± 3.5 | — | 9.8 ± 3.1 | — | |
| G106R1/G155R1 | pH 6 | 8.5 ± 1.0 | 14.7 ± 1.9 | 8.5 ± 1.1 | 14.7 ± 1.9 |
| pH 9 | 8.1 ± 1.0 | 15.7 ± 2.2 | 8.2 ± 1.9 | 15.7 ± 2.1 | |
The distances of nonilluminated and illuminated states under pH 6 and pH 9 were calculated from data in Fig. 3 by Fourier deconvolution (see Materials and Methods).