Figure 1.

Mean-squared atomic displacement, 〈r²〉, as a function of temperature for (A) tRNA and (B) protein lysozyme (data from Roh et al. (3,39)) at different levels of hydration. The accurate 〈r²〉 of biomolecules hydrated more than 0.70 h for tRNA and 0.50 h for lysozyme was not obtained due to crystallization of bulk D₂O (3,39). Insets show the additional hydration-induced contribution to the mean-squared displacements = 〈r²〉_Add = 〈r²〉 − 〈r²〉_Dry.