Table 1.

Effect of PLL length on the residence time τ_D of Rh-PE

Number		N	τD (μs)	DM (μm2/s)
1	Control	0	777 ± 39	8.1 ± 0.4
2	L-lysine	1	735 ± 37	8.5 ± 0.4
3	Pentalysine	5	1176 ± 59	5.4 ± 0.3
4	PLL	20	1870 ± 94	3.4 ± 0.2
5	PLL (unilateral)	126	1763 ± 88	3.6 ± 0.2
6	+PLL (bilateral)	126	3576 ± 179	1.8 ± 0.1
7	+1 M KCl	126	1278 ± 64	4.9 ± 0.2

Except number 5, PLL was present in both compartments. Numbers 5–7 denote subsequent additions. The conditions were as noted in Fig. 1.

PLL, poly-lysine; τ_D, residence time in the focus; Rh-PE, rhodamine labeled phosphatidylethanolamine; N, number of lysine units; D_M, lipid diffusion coefficient.