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. 2009 Jul 8;97(1):164–172. doi: 10.1016/j.bpj.2009.04.039

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Background-subtracted power spectra of strongly perturbed vesicles grouped by defocus (ΔZ₁ 1.4–2.1 μm; ΔZ₂ 2.2–2.8 μm; and ΔZ₃ 2.9–3.5 μm). Despite the large difference in defocus, no significant peak position shifts occur, implying that our data is not sensitive to the defocus parameter in CTF.