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. 2009 Jul 8;97(1):388–396. doi: 10.1016/j.bpj.2009.04.022

Figure 3.

Figure 3

nAChR. (A) Solution exchange protocol and transient currents during rapid solution exchange on a SSM with adsorbed membrane vesicles containing the nAChR. The positive transient current indicated by the arrow corresponds to the CCh induced Na+ inflow into the membrane vesicles. Basic buffer in all solutions: 30 mM Tris(HCl) pH 7.5, 3 mM EDTA, 1 mM EGTA. In addition, the different solutions contained: 100 mM NMGCl (NMG), 100 mM NaCl (Na), 100 mM NaCl, and 100 μM CCh (Na + CCh). Before the experiment the membrane vesicles were filled with 100 mM NMGCl. (B) Inhibition of the transient currents by the nAChR specific antagonist BTX. Each data point represents three to five independent experiments and error bars (SE) are given. The peak currents for each experiment were normalized to the control value obtained in the absence of BTX. The solid line is a hyperbolic fit to the data.