Table 5.
Lifetimes, preexponential factors, and average lifetimes as a function of pH for the transient species formed upon cutinase excitation
| Wavelength/species | Molecule | pH | Mean lifetime | Lifetimes and preexponential factors for global fitting |
R2 | ||
|---|---|---|---|---|---|---|---|
| τ1 (μs) | τ2 (μs) | τ3 (μs) | |||||
| 330 nm (0–50 μs data set) | Cutinase | 0.73 ± 0.06 | 14.8 ± 1.1 | 0.91229 | |||
| Trp· | 〈τ〉 | α1 | α2 | α3 | |||
| (μs) | |||||||
| 4.0 | 13.3 | 0.70 ± 0.10 | 0.30 ± 0.01 | ||||
| 8.5 | 13.1 | 0.74 ± 0.10 | 0.26 ± 0.01 | ||||
| 10.0 | 13.3 | 0.70 ± 0.09 | 0.30 ± 0.01 | ||||
| τ1 (μs) | τ2 (μs) | τ3 (μs) | |||||
| 520 nm (0–50 μs data set) | Cutinase | 0.69 ± 0.03 | 2,5 ± 0.08 | 0.97832 | |||
| Trp· | 〈τ〉 | α1 | α2 | α3 | |||
| (μs) | |||||||
| 4.0 | 1.0 | 0.95 ± 0.08 | 0.06 ± 0.06 | ||||
| 8.5 | 1.5 | 0.81 ± 0.02 | 0.19 ± 0.02 | ||||
| 10.0 | 2.4 | 0.10 ± 0.03 | 0.90 ± 0.03 | ||||
| τ1 (μs) | τ2 (μs) | τ3 (μs) | |||||
| 310 nm (0–1 ms data set) | Cutinase | 3.76 ± 0.20 | 48.8 ± 4.9 | 398 ± 40 | 0.99131 | ||
| Trp· | 〈τ〉 | α1 | α2 | α3 | |||
| (μs) | |||||||
| 10.0 | 368 | 0.43 ± 0.01 | 0.23 ± 0.01 | 0.35 ± 0.01 | |||
| τ1 (μs) | τ2 (μs) | τ3 (μs) | |||||
| 500 nm (0–1 ms data set) | Cutinase | 1.95 ± 0.03 | 25.0 ± 2.7 | 330 ± 26 | 0.98486 | ||
| Trp· | 〈τ〉 | α1 | α2 | α3 | |||
| (μs) | |||||||
| 4.0 | 316 | 0.21 ± 0.03 | 0.50 ± 0.01 | 0.30 ± 0.01 | |||
| 10.0 | 304 | 0.85 ± 0.01 | 0.10 ± 0.00 | 0.05 ± 0.00 | |||
| 310 nm (0–1 ms data set) | Cutinase | τ1 (μs) | τ2 (μs) | τ3 (μs) | |||
| Trp· | 3.76 ± 0.31 | 50 ± 7.8 | 400 ± 60 | 0.97841 | |||
| Nonirradiated | 〈τ〉 | α1 | α2 | α3 | |||
| (μs) | 0.43 ± 0.02 | 0.23 ± 0.02 | 0.35 ± 0.02 | ||||
| Irradiated | 10.0 | 370 | τ1 (μs) | τ2 (μs) | τ3 (μs) | 0.87159 | |
| 10.0 | 206 | 3.86 ± 0.42 | 210 ± 20 | ||||
| α1 | α2 | ||||||
| 0.54 ± 0.01 | 0.46 ± 0.01 | ||||||
| 400 nm (0–1 ms data set) | Cutinase | τ1 (μs) | τ2 (μs) | τ3 (μs) | |||
| RSSR·− | 1.83 ± 0.32 | 10.0 ± 2.0 | 390 ± 40 | ||||
| Nonirradiated | 〈τ〉 | α1 | α2 | α3 | 0.94340 | ||
| (μs) | 0.59 ± 0.08 | 0.18 ± 0.03 | 0.23 ± 0.01 | ||||
| 385 | τ1 (μs) | τ2 (μs) | τ3 (μs) | ||||
| Irradiated | 10.0 | 341 | 2.07 ± 0.17 | 30.0 ± 3.7 | 360 ± 30 | 0.97312 | |
| α1 | α2 | α3 | |||||
| 0.58 ± 0.05 | 0.15 ± 0.01 | 0.27 ± 0.01 | |||||