Table 1.
Kinetics of dye association and departitioning with liposomes (30% PE/70% PC, diameter: 100 nm, pH 7.4, 22.0°C)
| FM1-84 | FM1-43 | SynaptoGreen C3 | FM2-10 | FM4-64 | FM5-95 | SGC5 | |
|---|---|---|---|---|---|---|---|
| kon (nM−1 s−1) | 28 ± 2 | 18 ± 5 | 14 ± 4 | 5 ± 2 | 10 ± 3 | 9 ± 3 | 11 ± 1 |
| koff (s−1) | 63 ± 7 | 245 ± 18 | 204 ± 25 | 157 ± 30 | 240 ± 32 | 251 ± 28 | 178 ± 6 |
| Kd (nM) | 2.3 | 13.3 | 14.6 | 30.0 | 24.3 | 26.5 | 15.8 |
| kdiss (s−1) | 28 ± 1 | 126 ± 9 | 149 ± 22 | 174 ± 35 | 129 ± 20 | 131 ± 18 | 64 ± 2 |
| τdiss (ms) | 36.3 ± 0.9 | 8.0 ± 0.6 | 7.0 ± 0.9 | 6.4 ± 1.8 | 8.1 ± 1.2 | 7.9 ± 1.0 | 15.5 ± 0.4 |