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Effects of His-tag on membrane binding and lipid extraction monitored by time course fluorescence intensity at 518 nm from 10 μM GM2AP (solid) or 10 μM GM2AP10His-tag (open) with 50 μM POPC:DDHPE (9:1) in different conditions. (A) Results obtained for 50 mM NaOAc, pH 4.8, (B) 50 mM NaOAc, pH 6.4; (C) 50 mM NaOAc, 100 mM NaCl, pH 4.8. Results are discussed in the text. Excitation wavelength was 280 nm.
