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(A) Proximity and bifurcating hydrogen bonding between H562, T618, and S621. (B) Disrupted hydrogen bonding between H562, I618, and S621. (C) Disrupted orientation of W562 in the presence of T618 and S621. (D) The reestablished connectivity between W562, I618, and S621. (E) The disrupted H562 orientation with altered selectivity filter in S621. (F) Persistently disrupted selectivity filter in S621 in the presence of W562.
