Table 1.
Time constants of exponential components used to reproduce the transient absorption signals of wild-type NpHR and the T218V mutant
k0−1/ns | k1−1/ns | k2−1/μs | k3−1/μs | k4−1/ms | k5−1/ms | k6−1/ms | k7−1/ms | |
---|---|---|---|---|---|---|---|---|
WT 300 mM chloride | N | 550 ± 80 | 290 ± 80 | 560 ± 60 | 1.4 ± 0.1 | 17 ± 5 | 370 ± 30 | N |
WT 4 M chloride | 120 ± 30 | 600 ± 180 | 200 ± 40 | 500 ± 300 | 1.0 ± 0.2 | 3.7 ± 0.7 | 100 ± 80 | 120 ± 90 |
T218V 300 mM chloride | N | 640 ± 110 | 41 ± 25 | 620 ± 40 | 1.25 ± 0.05 | 5.23 ± 0.05 | 31 ± 5 | 88 ± 9 |
N, not observed; WT, wild-type.