Figure 2.

Kinetics of fibril formation by HEWL, monitored in situ with DLS. (A) Temporal evolution of field autocorrelation functions g₁(τ) of light scattered from HEWL solutions during amyloid fibrillogenesis at pH 2.0 and T = 50°C. After a latency period of ∼10 h, a prominent shoulder of slower decay rates emerges in correlation functions. (B) Semilog plot of PSDs derived from correlation functions in A. Initially, the PSD has a single, narrow, “small-aggregate” peak. After a lag period of several hours, a second “large-aggregate” peak emerges and starts to dominate PSDs. (C) Log-log plot of center position of two aggregate peaks in B as a function of incubation period. Note nucleation and gradual growth of larger aggregate peak. (D) Log-log plot of changes in static scattering intensity I_scat versus incubation period. Intensity values were corrected for changes in laser attenuation used to avoid detector saturation.