Table 2.

Comparison of measured (DLS) and estimated (AFM) hydrodynamic radii of intermediate aggregates

Sample	DLS Rh (nm)	AFM Rh (nm)
Monomer (0 h)	1.9	1.8 ± 0.3
Oligomer (3 h)	2.3∗	3.8 ± 0.4
Protofibril (10.5 h)	21.6	18.2 ± 3.0

DLS R_h (nm), measured hydrodynamic radii for lysoyzme monomers (0 h), weighted averages of monomers and oligomers (3 h), and protofibrils shortly after nucleation (10.5 h). AFM R_h (nm), predicted hydrodynamic radii for lysozyme monomers, oligomers, and protofibrils, based on AFM dimensions.

^∗DLS “oligomer radius” is average of monomers and oligomer radius, weighted by scattering intensity for each species present at 3 h.