Table 1.
Oligonucleotide | Sequence (5′- 3′) |
---|---|
Δ(9, 10): TS | TCAGAAAATTCACAAGCAGCCAATTCAATGTAGACAGACG |
Δ(9, 10): CS | GCTGCTTGTGAATTTTCTGATTTTTTTTTT-C3-SH |
Δ(9, 10): PS | SH-C6-TTTTTTTTTTCGTCTGTCTACATTGAATTG |
Δ(9, 10): sense primer | GCAGGAAACCAGTCTCAGTG |
Δ(9, 10): anti-sense primer | TTCACAAGCAGCCAATTCAATG (amplicon size: 118 bp) |
β-actin: sense primer | CATGTACGTTGCTATCCAGGC |
β-actin: anti-sense primer | CTCCTTAATGTCACGCACGAT (amplicon size: 250 bp) |
TS: target strand; CS: capturing strand; PS: probing strand.